Laboratory Assistant I

NCIRESan Francisco, CA, USA

30+ days ago

Salary

$18.48–$21.42 hourly

Job type

Part-time

Job description

Job Details

Description

The Atherosclerosis Research Lab within the UCSF Department of Surgery is offering an entry-level research assistant position. This opportunity is intended for a senior or recent graduate who is interested in gaining laboratory experience in preparation for entry into a health professional program.

Our laboratory has pioneered the study of extracellular vesicles and exosomes produced by macrophages as modulators of cardiometabolic diseases including diabetes, obesity and atherosclerosis. Ongoing projects in the lab aim to study the role of Diabetes and the APOE4 gene in altering macrophage-exosome biology and the impact this has on accelerating atherosclerosis, cardiac dysfunction and neurodegeneration in experimental mouse models. We are also developing new methods to produce exosomes that can serve to control cardiovascular disease and diabetes as next generation biologic medicines.

The successful applicant will assist senior lab staff with studies of murine-based projects that involve techniques such as exosome production and isolation, western blot, RNA / DNA extraction, PCR, flow cytometry, IHC, ultracentrifugation, cell culture, mouse surgery and husbandry.

Our prior trainees typically dedicated for 2-4 years of effort to our program before moving on to health professional programs with multiple publications credited to their names.

Examples of our recent publications can be found online :

PMID :

Essential Functions

Assist Pl and senior staff with routine laboratory upkeep.
Assist senior staff with transgenic mouse breeding and PCR genotyping.
Assist senior staff with routine gel electrophoresis preparation and Western blot of proteins
Assist senior staff with routine Exosome production and isolation from cultured cells and biofluids, and their study in cell signaling experiments
Assist senior staff with the harvest of mouse tissues and plasma along with the isolation of hematopoietic cells and lipoproteins using centrifugation and flow cytometry

Job Requirements

BS in molecular and cellular biology and biochemistry, or in final year of a BS program

Experience with methods of RNA / DNA and protein isolation and study using biochemical methods

Ability to work collaboratively in a team setting and assist with the training of junior staff as required

Strong oral / written communication, interpersonal and organizational skills.

Accuracy and attention to detail

Computer literacy with emphasis on Windows Excel, Adobe Photoshop for data analysis and its graphical output.

The base salary range for this position is $18.48 - $21.42 per hour. The base salary actually offered to a successful candidate will take into account various relevant and non-discriminatory business factors including, without limitation, the candidate’s geographic location, job-related experience, knowledge, and skills, and education, as well as internal equity considerations. A successful candidate may also be eligible to earn additional compensation including bonuses.

NCIRE is an Equal Opportunity Employer. This position requires access to United States Government research under the Veterans Administration. Per Veterans Administration regulations surrounding access to US research, qualified US Citizens will be selected over other individuals eligible to work in the United States. If you are eligible to work in the United States, you may still be considered for this position but only if no qualified US citizens apply